|
KMID : 0603520010060020092
|
|
Journal of Korean Association of Cancer Prevention
2001 Volume.6 No. 2 p.92 ~ p.97
|
|
|
Conditions of Single Cell Gel Electrophoresis of Chinese Hamster Ovary Cell for Detect the Genetic Toxicity of 2-aminoanthracene
|
|
Park Chang-Hwan
Kim Dong-Won
|
|
|
Abstract
|
|
|
|
The single cell gel electrophoresis (SCGE, comet assay) is becoming established as a genotoxicity test with manifold applications in vitro and in vivo. While the underlying principles are identical, various modifications of the method are in use which clearly affect the sensitivity and resolving power of the assay. We therefore performed SCGE experiments with CHO.1A2BRN2E cells, which express human cytochrome p450 1A2 cytochrome p450 reductase and N-acetyltransferase 2 genes, using different unwinding time, voltage/ampere, electrophoresis time and pH of electrophoresis buffer. DNA damage was induced by 2-aminoanthracene (2-AA) during 24 hours. On the basis of results obtained in this study, best SCGE conditions for detect DNA damage induced 2-AA to CHO.1A2BRN2E cells are 30 minutes unwindings, 25 volt/ 300 ㎃, 20 minutes electrophoresis with alkali electrophoresis buffer (pH 13.5).
|
|
|
KEYWORD
|
|
|
Single cell gel electrophoresis (SCGE), CHO cell, Metabolic activation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|